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Amaxa cell line nucleofector kit r
Cell Line Nucleofector Kit R, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+line+nucleofector%C2%AE+kit+r/electroporation/pm40733185-146-22-32
Average 86 stars, based on 1 article reviews
cell line nucleofector kit r - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Transfection:

Article Title: Chimeric antigen receptor specific to b-cell maturation antigen, a recombinant expression vector and a method thereof
Article Snippet: Amaxa® Cell Line Nucleofector® Kit R is used for electroporation of NK-92 cells (Lonza, Walkersville, Md.).

Article Title: A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy
Article Snippet: HeLa cells were transfected with small interfering RNA molecules specifically targeting RCK using the Amaxa® Cell Line Nucleofector® Kit R in conjunction with the NucleofectorTM System (Lonzo).

Article Title: Differential effects of polymorphisms in the 5' flanking region of IL12RB2 on NK- and T-cell activity.
Article Snippet: Single nucleotide polymorphisms (SNPs) in the 5′ flanking region of IL12RB2 are frequently detected in lep romatous leprosy patients, and may be possible immunogenetic factors that reduce transcriptional activity of the IL 12Rβ2 gene in Jurkat T cells.. This study determined the functional effects of these SNPs on NK cell activity, including IFN γ production and IL 12Rβ2 gene expression.. Reporter gene assays using the NK cell line NK3.3 revealed that transcriptional activities of the variant haplotypes were significantly higher in the NK cell line, in contrast to our previous results in Jurkat T cells.

Article Title: CIZ1 regulates G1 length and the CDK threshold for initiation of DNA replication to prevent DNA replication stress
Article Snippet: Transfection Amaxa® Cell Line Nucleofector® Kit R was used with 1 microgram of plasmid following recommended procedures.

Article Title: The ETS family member GABPα modulates androgen receptor signalling and mediates an aggressive phenotype in prostate cancer
Article Snippet: Transfections with Amaxa and the Cell Line Nucleofector® Kit R were used according to the manufacturer's protocols and siRNA were used at 1 μM (SMARTpool ON-TARGET plus: GABPα siRNA #L-011662-00-0005; control siRNA #D-001810-0X, Dharmacon).

Plasmid Preparation:

Article Title: Chimeric antigen receptor specific to b-cell maturation antigen, a recombinant expression vector and a method thereof
Article Snippet: Amaxa® Cell Line Nucleofector® Kit R is used for electroporation of NK-92 cells (Lonza, Walkersville, Md.).

Article Title: A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy
Article Snippet: HeLa cells were transfected with small interfering RNA molecules specifically targeting RCK using the Amaxa® Cell Line Nucleofector® Kit R in conjunction with the NucleofectorTM System (Lonzo).

Article Title: Differential effects of polymorphisms in the 5' flanking region of IL12RB2 on NK- and T-cell activity.
Article Snippet: Single nucleotide polymorphisms (SNPs) in the 5′ flanking region of IL12RB2 are frequently detected in lep romatous leprosy patients, and may be possible immunogenetic factors that reduce transcriptional activity of the IL 12Rβ2 gene in Jurkat T cells.. This study determined the functional effects of these SNPs on NK cell activity, including IFN γ production and IL 12Rβ2 gene expression.. Reporter gene assays using the NK cell line NK3.3 revealed that transcriptional activities of the variant haplotypes were significantly higher in the NK cell line, in contrast to our previous results in Jurkat T cells.

Article Title: CIZ1 regulates G1 length and the CDK threshold for initiation of DNA replication to prevent DNA replication stress
Article Snippet: Transfection Amaxa® Cell Line Nucleofector® Kit R was used with 1 microgram of plasmid following recommended procedures.

Article Title: The ETS family member GABPα modulates androgen receptor signalling and mediates an aggressive phenotype in prostate cancer
Article Snippet: Transfections with Amaxa and the Cell Line Nucleofector® Kit R were used according to the manufacturer's protocols and siRNA were used at 1 μM (SMARTpool ON-TARGET plus: GABPα siRNA #L-011662-00-0005; control siRNA #D-001810-0X, Dharmacon).

Small Interfering RNA:

Article Title: Chimeric antigen receptor specific to b-cell maturation antigen, a recombinant expression vector and a method thereof
Article Snippet: Amaxa® Cell Line Nucleofector® Kit R is used for electroporation of NK-92 cells (Lonza, Walkersville, Md.).

Article Title: A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy
Article Snippet: HeLa cells were transfected with small interfering RNA molecules specifically targeting RCK using the Amaxa® Cell Line Nucleofector® Kit R in conjunction with the NucleofectorTM System (Lonzo).

Article Title: Differential effects of polymorphisms in the 5' flanking region of IL12RB2 on NK- and T-cell activity.
Article Snippet: Single nucleotide polymorphisms (SNPs) in the 5′ flanking region of IL12RB2 are frequently detected in lep romatous leprosy patients, and may be possible immunogenetic factors that reduce transcriptional activity of the IL 12Rβ2 gene in Jurkat T cells.. This study determined the functional effects of these SNPs on NK cell activity, including IFN γ production and IL 12Rβ2 gene expression.. Reporter gene assays using the NK cell line NK3.3 revealed that transcriptional activities of the variant haplotypes were significantly higher in the NK cell line, in contrast to our previous results in Jurkat T cells.

Article Title: CIZ1 regulates G1 length and the CDK threshold for initiation of DNA replication to prevent DNA replication stress
Article Snippet: Transfection Amaxa® Cell Line Nucleofector® Kit R was used with 1 microgram of plasmid following recommended procedures.

Article Title: The ETS family member GABPα modulates androgen receptor signalling and mediates an aggressive phenotype in prostate cancer
Article Snippet: Transfections with Amaxa and the Cell Line Nucleofector® Kit R were used according to the manufacturer's protocols and siRNA were used at 1 μM (SMARTpool ON-TARGET plus: GABPα siRNA #L-011662-00-0005; control siRNA #D-001810-0X, Dharmacon).

Control:

Article Title: Chimeric antigen receptor specific to b-cell maturation antigen, a recombinant expression vector and a method thereof
Article Snippet: Amaxa® Cell Line Nucleofector® Kit R is used for electroporation of NK-92 cells (Lonza, Walkersville, Md.).

Article Title: A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy
Article Snippet: HeLa cells were transfected with small interfering RNA molecules specifically targeting RCK using the Amaxa® Cell Line Nucleofector® Kit R in conjunction with the NucleofectorTM System (Lonzo).

Article Title: Differential effects of polymorphisms in the 5' flanking region of IL12RB2 on NK- and T-cell activity.
Article Snippet: Single nucleotide polymorphisms (SNPs) in the 5′ flanking region of IL12RB2 are frequently detected in lep romatous leprosy patients, and may be possible immunogenetic factors that reduce transcriptional activity of the IL 12Rβ2 gene in Jurkat T cells.. This study determined the functional effects of these SNPs on NK cell activity, including IFN γ production and IL 12Rβ2 gene expression.. Reporter gene assays using the NK cell line NK3.3 revealed that transcriptional activities of the variant haplotypes were significantly higher in the NK cell line, in contrast to our previous results in Jurkat T cells.

Article Title: CIZ1 regulates G1 length and the CDK threshold for initiation of DNA replication to prevent DNA replication stress
Article Snippet: Transfection Amaxa® Cell Line Nucleofector® Kit R was used with 1 microgram of plasmid following recommended procedures.

Article Title: The ETS family member GABPα modulates androgen receptor signalling and mediates an aggressive phenotype in prostate cancer
Article Snippet: Transfections with Amaxa and the Cell Line Nucleofector® Kit R were used according to the manufacturer's protocols and siRNA were used at 1 μM (SMARTpool ON-TARGET plus: GABPα siRNA #L-011662-00-0005; control siRNA #D-001810-0X, Dharmacon).



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Image Search Results


Journal: Cell reports

Article Title: The Golgi complex governs natural killer cell lytic granule positioning to promote directionality in cytotoxicity

doi: 10.1016/j.celrep.2024.115156

Figure Lengend Snippet:

Article Snippet: Cell Line Nucleofector TM Kit R , Lonza , Ca# VCA-1001.

Techniques: Recombinant, Magnetic Cell Separation, Virus, Staining, Cell Culture, Plasmid Preparation, Transduction, Sequencing, Generated, Software